Composite

Part:BBa_K2957037

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-20)


hEF1a, CD19

Contents: hEF1a, CD19, SynthpA, with Amp resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and CD19 gene.

What does it do?: We used this part in transfection tests and for developing our synNotch system. It was given to us by Shiva Razavi, who designed and cloned the plasmid. If you would like the complete correct sequence please contact igem-2019-students@mit.edu and igem-2019-mentors@mit.edu

How to use it?: This part was used for transfection into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (a href=https://2019.igem.org/Team:MIT/Parts)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1880
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1373
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1880
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1880
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1542
    Illegal SapI site found at 1058
    Illegal SapI.rc site found at 876


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